Molecular detection of Stx1 and Stx2 genes of E. coli isolated from sub-clinical bovine mastitis in Mosul city

Subclinical mastitis in cattle is a pathological condition that causes financial burden on the dairy industry. In addition, it puts public sector at risk when pathogens of animal origin are present unpasteurized milk and products may enter human food chain cause serious illnesses. Our study aimed to determine percentage pathogenic Escherichia coli causing subclinical cows Mosul city by confirming existence Stx1 Stx2 genes. Eighty samples were obtained from suffering using sterile procedures November 2018 October 2019. was isolated, characterized, confirmed culture PCR. According our research, 36.3% (29/80). Moreover, molecular screening for specific gene revealed all isolates 100% carried uidA gene. PCR 27/29 (93.1%) had both + genes, possessed No significant relationship found between E. seasons. Even though there more winter than summer, this increase not statistically significant. This study's findings help pay attention one leading mastitis, which benefits private controlling disease.